任选9场

任选9场

超级ELISA优化方案-体外诊断试剂

时间:2019-08-06 08:50    浏览:

超级ELISA优化方案-体外诊断试剂

 

FANGFADEJIANLI

任选9场 1 KANGTIDEMEIBIAOJIJIZHILIANGJIANDING

任选9场 BENSHIYANCAIYONGGAILIANGGUODIANSUANNAFAJIANGLAGENGUOYANGHUAWUMEIBIAOJIZAIKANGTISHANG[1],BIAOJIWANHOUQUSHANGQINGYONGSephedex G200 NINGJIAOCENGXIJINXINGCHUNHUA,XITUOYEWEI0.2mol/L pH7.4DEPBS,LIUSUWEI15ml/h,FENBUSHOUJI,MEIZHI3ml,YIZIWAIFENGUANGGUANGDUJICEA280XIGUANGDUZHI,YIXITUOTIJIWEIHENGZUOBIAO,XIGUANGZHIWEIZONGZUOBIAOZUOTU,SHOUJIDIYIFENGJIWEIMEIBIAOKANGTIFENG。

任选9场 BIAOJIWANHOUYONGZIWAIFENGUANGGUANGDUJIZAIFENBIEZAI280nm、JI403nmCHUCEDINGMEIBIAOJIWUDEAZHI,JISUANMIANYIQIUDANBAILIANG、MOERBIZHI、MEIJIEHELVYIJIBIAOJILV。

2 BAOBEIYONGHUANCHONGYEJIZUISHIBAOBEIKANGYUANNONGDUDEYOUHUA

2.1 BAOBEIHUANCHONGYEDEYOUHUA

任选9场 BAOBEIKANGYUANSHI,WEILEDEDAOZUIJIADEBAOBEIXIAOGUO,WOMENCAIYONGLETANSUANYANHUANCHONGYE(50mM ph9.6 )、LINSUANYANHUANCHONGYE(50mM ph7.6)、YIJITRISYANSUANHUANCHONGYE(50 mM ph7.6)SANZHONGHUANCHONGYEZUOWEIBAOBEIYE,KANGYUANBAOBEINONGDUWEI5ug/ml,JI2.5ug/ml,MEIBIAOKANGTIGONGZUONONGDUWEI1:400JI1:300,YONGZIDONGMEIBIAOYIFENXI,XUANZEZUIJIADEBAOBEIHUANCHONGYEXITONG(BIAO5)。TONGSHIWEILEBAOZHENGHUANCHONGYENENGGOUZHANGQIBAOCUN,WOMENZAIBAOBEIYEZHONGTIANJIALE0.01%DELIULIUGONGZUOWEIFANGFUJI。

2.2 ZUISHIKANGYUANBAOBEINONGDUDEYOUHUA

YONGYOUHUAHAODEBAOBEIHUANCHONGYE,JIANGBAOBEIKANGYUAN(ALB)XISHICHENG0.1ug/ml,1.0ug/ml,2.0ug/ml,4.0 ug/ml,6.0 ug/ml,8.0 ug/ml,10.0 ug/mlDENGLIUGENONGDU,BAOBEIWEIKONGBAN,MEIKONG100ul;JINGZHENGKANGYUANNONGDUWEI4.0 ug/ml,2.0 ug/ml;MEIBIAOKANGTIXISHIDUWEI1:300,JINGFUYU、XIANSE、ZHONGZHIHOUYONGZIDONGMEIBIAOYIFENXI(BIAO6)。XIANSEDEQIANGDUZAIYIDINGFANWEINEIYUBAOBEIDEKANGYUANLIANGCHENGZHENGBI,DANSUIZHEBAOBEIKANGYUANNONGDUDEZENGJIA,XIANSEDEQIANGDUFANERJIANGDI,WOMENXUANZELINJIEBAOBEIZHIZUOWEIBAOBEIKANGYUANLIANG。

任选9场 3 FENGBIYE、XIDIYEJIBAOHUYEDEYOUHUA

1 FENGBIYEDEYOUHUA

任选9场 CAIYONGWENXIANBAODAODEFANGFAJINXINGFENGBI,QIFENGBIYEDEZUCHENGWEI:10mMLINSUANYANHUANCHONGYEHAN1%DENIUXUEQINGBAIDANBAI(BSA)。

2 XIDIYEDEPEIZHI

CAIYONGWENXIANBAODAODEFANGFAPEIZHIXIDIYE。

2 MEIBIAOBANBAOHUYEDEYOUHUA

任选9场 MEIBIAOBANJINGGUOFENGBIHOUZAIDIWENXIAJINNENGCUNFANG2ZHOUZUOYOU,WEILEYANZHANGGUXIANGMEIBIAOBANSHANGKANGYUANDEWENDINGXING,WOMENZENGJIALEYIBUBAOHUMEIBIAOBANDECHENGXU。ZAI10mMDELINSUANYANZHONGJIARUSHILIANGDETANG、WUGUANDANBAIZHI、QINGDAMEISUYIJIERJIAJINSHULIZI,ZUOWEIMEIBIAOBANBAOHUJI,ZAIFENGBIWANHOUJIARUBAOHUYEYU4℃BINGXIANGZHONGFUYUGUOYE,TONGSHIYUWEIZUOBAOHUDEMEIBIAOBANJINXINGDUIZHAO,RANHOUJIANGBAOHUDEJIWEIBAOHUDEMEIBIAOBANZHIYU37HONGXIANGZHONGFANGZHI15TIAN,KAOCHABAOHUYEDUIMEIBIAOBANDEWENDINGXINGDEYINGXIANG。

任选9场 4 MEIBIAOKANGTIXISHIDUDEYOUHUA

任选9场 KANGYUANBAOBEINONGDUWEI4ug/ml,JIANGMEIBIAOKANGTIZUOXILIEXISHI:1:100,1:200;1:300;1:400;1:500;1:600;1:1000;JINGFUYU、XIDIHOU、XIANSEZHONGZHIHOU,YONGZIDONGMEIBIAOYIFENXI(BIAO7),XUANZEAZHIWEI1.5ZUOYOUDEMEIBIAOKANGTIXISHIDUWEIZUISHIGONGZUONONGDU。

5 MEIBIAOKANGTIBAOHUYEDEYOUHUA

任选9场 DINONGDUDEMEIBIAOKANGTIJIRONGYISHOUDAOGAOWEN、WEISHENGWU、YIJIDANBAIMEIDEYINGXIANGERSHIHUO,YANZHONGYINGXIANGCHANPINDEHUOJIAQI,GUOQUTONGCHANGJIANGMEIBIAOKANGTIDONGGANBAOCUN,RANERZHEIYANGDEBAOCUN,BUJINYOUYUZAIDONGGANDEGUOCHENGZHONGHUIYINGXIANGMEIBIAOKANGTIDEHUOXING,ERQIEZAILINCHUANGYINGYONGZHONGPOWEIBUBIAN。SHIDANGDEHUANCHONGYEYIJITIANJIAWUGUANDANBAIZHI、TANG、FANGFUJIYIJIYIXIEHANANJIDEHUAHEWUDENGDOUHUIDUIDUIMEIBIAOKANGTIDEHUOXINGQIYIDINGDEBAOHUZUOYONG,YINCIWOMENSHEJILEYIZUBAOHUJIYONGYUBAOHUMEIBIAOKANGTI,YUWEITIANJIARENHETANGDANBAIDEMEIBIAOKANGTIZUODUIZHAO,JIANGJINGBAOHUDEMEIBIAOKANGTIJIWEIBAOHUDEMEIBIAOKANGTIZHIYU37℃HONGXIANGZHONGFANGZHI6TIAN,KAOCHABAOHUYEDUIMEIBIAOKANGTIDEYINGXIANG。

6 DIWUYEDEYOUHUA

CHANGYONGDEZUOWEIMEILIANMIANYIXIFUSHIJIYOUOPDHETMBXITONG,YOUYUOPDYOUZHIAIDEWEIXIANXINGYIJIYONGQIANXUYAORONGJIEDENGZHUDUOQUEDIAN,YINCIWOMENXUANYONGTMBZUOWEIDIWUXITONG,CAIYONGWENXIANBAODAODEFANGFA:QUSHILIANGDETMBRONGJIEZAIDMSOZHONG,RANHOUJIARUSHILIANGDECHAOCHUNSHUI,PEIZHIZUOWEIDIWUBYE;RONGJIESHILIANGDEGUOYANGHUAQINGNIAOSUYU100mMLINSUAN-NINGMENGSUANHUANCHONGYEZHONG,PEIZHIZUOWEIDIWUAYE,YONGQIANJIANGDIWUAYEJIDIWUBYEDENGTIJIHUNHE。

ZAIWENXIANBAODAODEJICHUSHANG,WOMENSHIDANGDIAOZHENGLETMBDEYONGLIANGYIJIGUOYANGHUAQINGNIAOSUDEYONGLIANG,BINGJIARULEMEICUJINJI,YUWENXIANBAODAODEDIWUJINXINGBIJIAO。SHIYANFANGANWEI:JIANGHUOHUADELAGENGUOYANGHUAWUMEIFENBIEZUO1:1000;1:10000;1:100000;1:200000;1:400000;1:800000DENGXILIEXISHI,BIJIAOLIANGZHONGBUTONGPEIFANGDEDIWUDELINGMINDU(BIAO10)。

7 BIAOZHUNPINJIYANGPINXISHIYE

WOMENCAIYONG10mMDELINSUANYANHUANCHONGYENEIHAN0.5%DEBSAZUOWEIBIAOZHUNPINJIYANGPINDEXISHIYE。

8 KANGYUANKANGTIFANYINGSHIJIANDEYOUHUA

KANGYUANKANGTIFANYINGHUISUIZHESHIJIANDEZENGJIA,FANYINGLIANGYEZENGJIA,DANDADAOYIDINGDESHIJIANHOU,FANYINGJIDADAOPINGHENG,WOMENQUFANYING10min,20min,30min,40min,50min,60min,

70min,90minDENG8GEDIANJINXINGBIJIAO(BIAO11),YIQUEDINGZUIJIAKANGYUANKANGTIFANYINGSHIJIAN。

9 DIWUZUOYONGSHIJIANDEYOUHUA

ZAIQITATIAOJIANXIANGTONGDEQINGKUANGXIA,WOMENQU5min,10min,15min,20min,25min,30min,35min

40min,50min,60min,80minDENG11GESHIJIANDIANJINXINGBIJIAO(BIAO12),QUEDINGZUIJIADEDIWUZUOYONGSHIJIAN。

任选9场 10 JILIANGFANYINGQUXIANDEJIANLIJIQUXIANNIHEFANGSHIDEQUEDING

任选9场 ZAIQITACANSHUJUNYIJINGYOUHUAWANCHENGDEQINGKUANGXIA,JIANGKANGYUANBIAOZHUNPINYONGBIAOZHUNPINXISHIYEYICIXISHIWEI:160mg/L,80mg/L,40mg/L,20mg/L,10mg/L,5mg/L,2.5mg/L,1.25mg/L,0.625mg/L,

0.3125 mg/L,0.156 mg/L,0.780 mg/LDENG12GEXISHIDU,TONGSHISHEZHIYINXINGHEKONGBAIDUIZHAO,JIARUYIBAOBEIHAOKANGYUANDEMEIBIAOBANZHONG,TONGSHIJIARUMEIBIAOKANGTI,JINGFUYU、XIANSE、ZHONGZHIHOUYONGZIDONGMEIBIAOYIFENXI(BIAO12),CAIYONGWENXIANBAODAODE4PDUISHUNIHEQUXIAN

 

                                                                                                                             南京卡米洛生物工程有限公司

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