任选9场

任选9场

ELISA中非特异性显色原因

时间:2019-07-31 17:33    浏览:

ELISA中非特异性显色原因分析

 

任选9场 【ZHAIYAO】:YINGXIANGELISAZHONGFEITEYIXINGXIANSEDEYUANYINHENDUO,RUSHIJIHETEYIXING、JIANYANBIAOBENZHONGHANMEIBIAOJIWUDEGANRAOWU,CAOZUOGUOCHENGZHONGDEWENTI。BENWENJIUZHEIYIYUANYINZUOYIJIANYAOFENXI,YIBIANKEYITONGGUOYISHANGCUOSHIBAFEITEYIXIANSEJIANGZHIZUIDIXIANDU,CONGERTIGAOJIANCEDETEYIXING,BINGDEDAOGENGZHUNQUE、KEKAODESHIYANJIEGUO。

【GUANJIANCI】 ELISA FEITEYIXING XIANSE

MEILIANMIANYIXIFUSHIYAN(ELISA)ZI1971NIANZIWENSHIYILAI,YIQIMINGANXINGGAO,TEYIXINGQIANG,CAOZUOJIANBIAN、ANQUAN,ERGUANGFANYINGYONGYULINCHUANGZHENDUAN,KAICHUANGLEMIANYIXUEZHENDUANDEXINJIYUAN。DANTONGQITAMIANYIZHENDUANFANGFAYIYANGMEIMIANSHIJIZAITADEYANJIU、SHENGCHANJISHIYONGZHONGCHANGCHANGHUIPENGDAOFEITEYIXINGWENTI,BENWENJIUZAISHIYANGUOCHENGZHONGCHANSHENGDEYIXIEYUANYINJIRUHECAIQUYIXIECUOSHI,XIAOCHUFEITEYIXINGXIANSEZUOYIFENXI。

1、SHIJIHETEYIXINGYINSU

任选9场 1、1 GUXIANGZAITIDEXUANZE。ELISAZHONGCHANGYONGDEGUXIANGZAITIYOUWEILIANGDIDINGBAN、XIAOZHUHEXIAOSHIGUANSANZHONG,YIWEILIANGDIDINGBANZUIWEICHANGJIAN[1]。TAJUYOULIANGHAODEXIFUXINGNENG,KONGDITOUMINGDUGAO,KONGBAIZHIDI,GEBANZHIJIAN、TONGYIBANGEKONGZHIJIANXINGNENGXIANGJIN。JUBENYIXIELISABANYOUYUYUANLIAODEBUTONGHEZHIZUOGONGYIDECHABIE,GECHANPINDEZHILIANGCHAYIHENDA。YINCI,ZAIXUANZESHIJIHETONGSHIYAODUIQISHIYONGDEWEIKONGBANXINGHAOJINXINGRENZHENG,PINGGU。

任选9场 1、2BAOBEIWUDECHUNDU。ZAIMEILIANMIANYICEDINGYOUQISHIJIANJIEELISAZHONG,SHIJIDETEYIXINGQUJUEYUSHIYONGKANGYUANDECHUNDU。MUQIANYOUYUJISHUTIAOJIANDEXIANZHI,BAOBEIYONGKANGYUANHUOKANGTIDECHUNDUBUKENENGDADAO100%,SUOYIYOUXIEFEITEYIXINGXIANSEBUKEBIMIAN,ZHINENGJINKENENGTIGAOCHUNDU,TIGAOTEYIXING。MUQIANSHIYONGDEBAOBEIKANGYUANYIBANWEIHECHENGDUOTAIKANGYUAN。

任选9场 1、3BAOBEIKANGTIDEXIAOJIA。JUYOUGAOQINHELIHEGAOTEYIXINGDEBAOBEIKANGTI,SHIJUEDINGSHIJITEYIXINGDEZHONGYAOFANGMIAN。

1、4 FENGBI。SHIELISAZHONGZHONGYAODEYIBU,MUDESHIFENGBIGUXIANGZAITIBIAOMIANSHANGWEIBEISUOZHANJUDEKONGXI[2],JIANSHAOHOUXUBUZHOUZHONGFEITEYIXINGDANBAIDEGANRAO。

2、JIANYANBIAOBENZHONGHANYOUMEIBIAOJIWUDEGANRAOWU

2、1NEIYUANXINGGANRAOWU:LEIFENGSHIYINZI(RF)、HUANGDANDENG,LEIFENGSHIYINZISHIKEZUOYONGYUDUOZHONGDONGWUYIJIRENIgGFcDUANDEZISHENKANGTI,DUOSHUWEIIgMLEI,NENGCHONGDANGKANGYUANCHENGFENYUGUXIANGJIMEIBIAOKANGTIFANYING,CONGERCHENGXIANFEITEYIXINGXIANSE。

HUANGDANXUEBIAOBENZHONGCHANGHANYOUNEIYUANXINGGUOYANGHUAWUMEI,RUYONGLAGENGUOYANGHUAWUMEIWEIBIAOJIWU,JIUYOUKENENGCHANSHENGFEITEYIXINGXIANSE。

2、2 WAIYUANXINGGANRAOWU,CHANGCHANGYINYANGPINCAIJI、ZHUCUN、CHULIBUDANGZAOCHENGRUYANGPINRONGXUE,BEIXIJUNWURAN,BIAOBENNINGJIBUQUANDENG。RONGXUEBIAOBEN,HONGXIBAORONGJIEPOLIE,SHIFANGCHUXUEHONGSUXINGCHENG,ERXUEHONGSUZHONGDETIEBULINSHIGUOYANGHUAWUMEIDELEISIWU,YIHRPWEIBIAOJIDEELISACEDINGZHONG,RONGXUEBIAOBENKENENGHUIZENGJIAFEITEYIXINGXIANSE。RUYOUXIJUNWURAN,JUNTIZHONGKENENGHANYOUNEIYUANXINGHRP(LAGENGUOYANGHUAMEI)[2],YOUGUONEIBAODAOMEIMIANHBsAgSHIJIJIANCERONGXUEBIAOBENSHIKEZAOCHENGJIAYANGXING[3]。RUZAIBINGXIANGZHONGBAOCUNGUOJIU,QIZHONGDEIgGKEFASHENGJUHE,ZAIJIANJIEFAELISAZHONGKESHIBENDIJIASHEN[2]。YINCI,XUEBIAOBENZAICAIJICHULISHIYINGXIAOXIN,ZAIBINGXIANGZHONGBAOCUNBUYIGUOJIU。

BIAOBENNINGJIBUQUANSHI,XUEQINGZHONGHUICANLIUBUFENXIANWEIDANBAIYUAN,YEYIZAOCHENGJIAYANGXING。

3、CAOZUOGUOCHENGZHONGDEWENTIZAOCHENGJIAYANGXING

3、1JIAYANG

DUIYUJIANJIEELISABIAOBENYIBANDOUYAOJINXINGXISHI,RUGUOJIAYANGBUZHUNJIUHUIZAOCHENGWUCHA,YOUQIDANGXISHIBEISHUDASHI,HENXIAODEJUEDUIWUCHA,HUIDAOZHIJIAODADEXIANGDUIWUCHA,SHIYINXING(HUORUOYANGXING)BIAOBENCHENGYANGXING(HUOYINXING)。JIAYANGSHIYINGJIANGSUOJIAWUJIAZAIELISABANKONGDEDIBU,BIMIANJIAZAIKONGBISHANGBU,BINGZHUYIBUKEJIANCHU,BUKECHANSHENGQIPAO。MUQIAN,DABUFENXUEZHANDOUYISHIYONGQUANZIDONGMEIMIANJIAYANGXITONGCHULIBIAOBEN,KEJIAOHAODIBIMIANYISHANGWUCHA。

3、2XIDI

ZAIELISAZHONGZHENGQUEDEXIDISHIBAOZHENGDEDAOKEZHONGFUJIEGUODEGUANJIANYIBU,YINGYINQICAOZUOZHEZHONGSHI。WULUNSHISHOUGONGCAOZUOHAISHIJIQICAOZUO,DECHUBUZHENGQUEDEJIEGUOCHANGYUBUZHENGQUEDEXIDIYOUGUAN,ELISAJIUSHIKAOXIDILAIDADAOFENLIYOULIHEJIEHEDEMEIBIAOJIWUDEMUDE。TONGGUOXIDIYIXIAOCHUCANLIUZAIBANKONGZHONGMEINENGYUGUTIKANGYUANHUOKANGTIJIEHEDEWUZHI,YIJIZAIFANYINGGUOCHENGZHONGFEITEYIXINGXIFUYUGUXIANGZAITIDEGANRAOWUZHI。

3、3 WENYU

MEIZHONGSHIJIDOUYOUQIZUIJIAFANYINGMOSHI,QIZHONGWENDUHEWENYUSHIJIANKONGZHISHIZHONGYAOYINSU。YINFUYUWENDUGAO,FANYINGSHIJIANZHANG,HUIZAOCHENGZHENGBANBENDIGAO,YANGXINGLVGAO。WENYUYIBANYONGSHIHEHUOSHUIYU,FANYINGBANBUYIDIEFANG,YIBAOZHENGGEBANWENDUDOUNENGXUNSUPINGHENG,WEIBIMIANZHENGFA,BANSHANGYINGJIAGAI。

3、4MEIBIAOYIPANDU

任选9场 ZUOWEIJILUCEDINGJIEGUODEYIQI,MEIBIAOYIDEXINGNENGWENDINGYUFOU,JUEDINGJIEGUODEKEKAODU。SHOUXIANMEIBIAOYIYINGDINGQIJINXINGBAOYANG,DUILVGUANGPIANYAODINGQIXIAOZHENG;QICIMEIBIAOYIBOZHANGSHEZHIYAOZHENGQUE,ZUIHAOSHIYONGSHUANGBOZHANG,YIGEJIANCEBOZHANG,YIGECANBIBOZHANG,YIXIAOCHUWEIKONGBANDIBUHUAHEN、BUPING、ZHIYINHUOYEMIANGAODUCHAYIZAOCHENGDEGUANGGANRAO。CIWAI,ZAIYONGMEIBIAOYIDUSHUSHIZUIHAOXIANCASHIWEIKONGBANDIBUBINGYAPINGBANTIAO。YOUYUGEZHONGMEIBIAOYIXINGNENGYOUSUOBUTONG,SHIYONGZHONGYINGXIANGXIYUEDUSHUOMINGSHU

任选9场 ZONGSHANGSUOSHU,YOUYUMEILIANMIANYIXIFUJISHUMUQIANZAIJISHUSHANGQUEFABIAOZHUNHUA,JINGUANMUQIANGUOJISHANGYIJIWOGUOYOULEBUFENBIAOZHUNXUEQINGHUOCANBIXUEQING(XUEQINGPAN)。DANYOUYUSHOUFANGFAXUEJIJISHUTIAOJIANDEXIANZHI,ZAIMEILIANXIFUCEDINGZHONGYOUSHIBUKEBIMIANDEHUICHUXIANYIDINGDEFEITEYIXING,DANWOMENKEYITONGGUOYISHANGCUOSHIBAFEITEYIXINGXIANSEJIANGZHIZUIDIXIANDI,CONGERTIGAOJIANCEDETEYIXING,BINGDEDAOGENGZHUNQUE、KEKAODESHIYANJIEGUO。

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